Objectives and competences
• Detailed knowledge of the structure, chemical composition and transport mechanisms in cell.
• To get insights into the mechanisms of signal perception and mechanisms of inter- and intracellular sensing.
• To recognise some adaptations to stress factors at the cellular level and defense mechanisms.
• Application of methods in environmental monitoring
Content (Syllabus outline)
I. Eukaryotic cell organisation:
Molecular compounds of the cell and the organization of prokaryotic, plant, fungal and animal cells. Experimental approaches. Endosimbyotic theory and evolution of life. Biological membranes and transport. Endomembranes: endoplasmatic reticulum and Golgi apparatus. Endocytosis and exocitosis. Organelles of energy transformations (mitochondria, peroxisomes, glyoxisomes). Cytosol. Cytoskeleton. Interphase nucleus. Cromatyin. Chromosome. Nuclear divisons: mitosis and meiosis. Programmed cell death. Cells of immune system. Specific plant components: cell wall, plastids and vacuoles.
II. The environmental impact on plant cell, cell types and cell compartments
Alterations in chemical compounds, enzyme activity and metabolism, changes of cell organelles, changes in membrane organization, DNA, programmed cell death.
III. Microscopical techniques in ecotoxicology and biomonitoring
Learning and teaching methods
Subject is based on lectures that give basic concepts of cell biology with the help of the audio-visual techniques.
The lectures are prerequest for enrolling in the laboratory courses.
Written exam after passing the colloquium, laboratory practicals, independent work.
Intended learning outcomes - knowledge and understanding
By the end of this course students should be able to:
Recognise the difference between plant and animal cell.
Identify organells at TEM micrographs.
Explain the structure and function of main compartments.
Recognise and understand the importance of plant cell wall and vacuole in defense against pathogens and pests.
Explain the role of plant cell wall and vacuole for organoleptic properties of fruits.
Understanding the physiological disorders on subcellular level.
Understanding the role of the programmed cell death for plant/animal/human organ structure and function.
Intended learning outcomes - transferable/key skills and other attributes
Readings
Pollard T. D. et al., Cell Biology,2016, Third Edition.
Kleinig H., Sitte P., Zellbiologie, 1999.
Tekoča periodika iz katere povzemam izročke:
The Plant Cell
Protoplasma
Plant Cell and Environment
Plant and Cell Physiology
Journal of Plant Biotechnology
Trends in Plant Science
Additional information on implementation and assessment 60%
10%
30%
Written exam
Seminar work
Colloquium after passing the lab courses