Objectives and competences
The objective of this course is for students to be able to recognize different sample preparation methods, choose appropriate procedures depending on measured compound and applied analytical method, recognize errors and interpret results.
Content (Syllabus outline)
• preparation of biological samples for chemical analysis (drying, grinding, extraction and acid digestion of samples)
• metal fractions in soil: total and bioavailable metal fractions
• volumetric methods: neutralization titrations (Kjeldahl method for total nitrogen), redox titrations (total carbon)
• spectrometric methods: MAS, AAS, AES ETAAS, ICP-AES, MP-AES
• CNS analyzer
• applications: determination of macro- and microelements, and heavy metals in food, feed, and soil samples
• chromatographic methods: gas chromatography, liquid chromatography
• applications: determination of pesticide residues, selected organic compounds in fruit and vegetables
Learning and teaching methods
Lectures and laboratory work.
Intended learning outcomes - knowledge and understanding
On completion of this course the student will be able to:
• point out the differences between different sample preparation methods and choose the appropriate depending on measured analyte choose and use suitable titration method for the determination of analyte in biological samples, understand the principles of spectroscopic methods (MAS, AAS, ETAAS, ICP-AES, MP-AES) and CNS analyzer, recognize differences between them and search for examples of their practical use
• recognize the advantages of modern analytical methods compared to classical procedures
• compare the principles of LC and GC separations and suggest appropriate separation technique for the determination of some organic compounds (sugars, acids, pigments, pesticide residues…)
• recognize errors and interpret analytical results
Intended learning outcomes - transferable/key skills and other attributes
- use of labware and equipment
- laboratory skills during experimenting in the lab
- communication skills: manner of expression at written examination and lab course
Readings
• Skoog, D. A., Holler, F. J., West, D. M. (1996). Fundamentals of analytical chemistry (7th ed.). Saunders.
• Kristl, J. (2022). Kemijske analizne metode v kmetijstvu in toksične snovi v ekosistemih: navodila za laboratorijske vaje (1. izd.). Univerza v Mariboru, Univerzitetna založba.
https://press.um.si/index.php/ump/catalog/book/654
• Paul, E. A., Clark, F. E. (1996). Soil microbiology and biochemistry (2nd ed.). Academic press. (poglavje 6)
Strokovno področna znanstvena periodika.
Prerequisits
Attendance at the exercises is mandatory to the extent of 80% of the scheduled hours.
Additional information on implementation and assessment laboratory work
written exam
