Objectives and competences
The objective of this course is for students to be able to recognize different sample preparation methods, choose appropriate procedure depending on measured compound and applied analytical method, recognize errors and interpret results.
Content (Syllabus outline)
• preparation of biological samples for chemical analysis (freeze-drying, extraction and acid digestion)
• volumetric methods (neutralization, complex-formation and redox titrations).
• spectrometric methods (MAS, AAS, AES ETAAS, ICP-AES) and their applications for the determination of inorganic and organic compounds, macro- and microelements, and heavy metals in food (fruit, vegetables, meat, milk, etc.), feeds and drinking water
• chromatographic methods (gas chromatography, liquid chromatography) and their applications for the determination of pesticide residues, plant pigments, antioxidants and organic acids in fruit and vegetables
Learning and teaching methods
Lectures and laboratory work.
Intended learning outcomes - knowledge and understanding
Knowledge and understanding:
On completion of this course the student will be able to:
• point out the differences between different sample preparation methods and choose the appropriate depending on measured analyte
- choose and use suitable titration method for the determination of analyte in biological samples
- understand the principles of spectroscopic methods (MAS, AAS, ETAAS, ICP-AES), recognize differences between them and search for examples of their practical use
• compare the principles of LC and GC separations and suggest appropriate separation technique for the determination of some organic compounds (sugars, acids, pigments, pesticide residues,…)
• recognize errors and interpret analytical results
Intended learning outcomes - transferable/key skills and other attributes
- use of labware and equipment
- laboratory skills during experimenting in the lab
- communication skills: manner of expression at written examination and lab course
Readings
• Douglas, A. Skoog, Donald M., West, F. James Holler. 1992. Analytical chemistry. Saunders College Publisher.
• Douglas A. Skoog, Donald M. West, F. James Holler, Stanley R. Crouch (2014), Fundamentals of Analytical Chemistry, 9. izdaja, Cengage Learning, ZDA,
• Kristl, J. (2022). Kemijske analitične metode v kmetijstvu in toksične snovi v ekosistemih: Navodila za laboratorijske vaje. Maribor: Univerzitetna založba.
Strokovno področna znanstvena periodika.
Additional information on implementation and assessment active attendance at lab work, writing lab reports 20 %
written exam 80 %
